TABLE 1.
Primer sequences used for RT-PCR and in situ hybridization, respectivelya
| Amplification product | Sequence | Nucleotides | Annealing temp (°C) |
|---|---|---|---|
| Inhibin alpha (fwd) | GTAAGCTTCATCAGGGCAAGTGAACTATGG | −16-1105 | 58 |
| Inhibin alpha (rev) | GATCTAGAGGTGCTTTTAGATACAAGCACAGTG | ||
| Activin betaA (fwd) | ATGTGGAGATAGAGGACGACATT | 298-1223 | 55 |
| Activin betaA (rev) | GATGTTTTGACCATCATCGTAATA | ||
| Activin betaB (fwd) | ATCGACTTTCGGCTCATCGG | 1360-1635 | 58 |
| Activin betaB (rev) | CACGATCATGTTGGGCACATC | ||
| ActRIA (fwd) | CTGGCCAAGCTGTGGAGTGCTGCCAA | 259-829 | 55 |
| ActRIA (rev) | GTACTGGAGTGTCTAGAGGTCATGT | ||
| ActRIB (fwd) | GGAAAGCTTATGGCGGAGTCGGCCGGA | −7-553 | 55 |
| ActRIB (rev) | GATCGTAGACGAGATCCTGGAGCGT | ||
| ActRIIA (fwd) | CGACGACATTGTTTTGCTACC | 168-455 | 55 |
| ActRIIA (rev) | CCCCGCAATTAACATAAGTGG | ||
| ActRIIB (fwd) | GGATGTATCGTCATCGGAAAC | 472-1239 | 55 |
| ActRIIB (rev) | CCATGGCGTACATGTCGATAC | ||
| GAPDH (fwd) | GAGATTGTTGCCATCAACGAC | 78-962 | 55 |
| GAPDH (rev) | CCTGTTGCTGTAGCCGTATTC | ||
| LH-R (fwd) | ATGGATCCCTCTCACCTATCTCCCTGT | 176-878 | 62 |
| LH-R (rev) | AGTCTAGATCTTTCTTCGGCAAATTCCTG | ||
| P450c17 (fwd) | CGAAGCTTGGAACTTGTGGGTCTCTTGC | 6-513 | 62 |
| P450c17 (rev) | CGCTCGAGAACCTCAACCTGTGCATCCT | ||
| P450arom (fwd) | GACACATCATGCTGGACACC | 575-1297 | 62 |
| P450arom (rev) | CAAAGCCAAAAGGCTGAAAG | ||
| T7-ActRIA (fwd) | GTAATACGACTCACTATAGGGAAAGCCGGTTATACAATGGTC | −14-437 | 58 |
| SP6-ActRIA (rev) | GCATTTAGGTGACACTATGATTGCCTCTCTTAAACTTCCTG | ||
| T7-ActRIIB (fwd) | GTAATACGACTCACTATAGGGAGATGACTTCAATTGCTACGAC | 237-496 | 58 |
| SP6-ActRIIB (rev) | GCATTTAGGTGACACTATGGAGGTTTCCGATGACGATAC |
a
Underlined sequences denote restriction sites or binding sites for RNA polymerases. Nucleotide positions are given relative to the start codon of the respective open reading frame. For further details, see text. fwd; forward; rev; reverse.