FIG. 5.

(A) Immunoblot analysis displaying ectopic expression of PIM3 in NIH 3T3 cells. This blot is representative of the levels of PIM3 expression in the cells used in the agar and tumorigenesis assays. (B) Agar assays of NIH 3T3 cells expressing PIM3, EWS/FLI1, and empty vector. Images were recorded at day 21 and are representative of three independent experiments. Colony numbers represent the number of macroscopic colonies present on each plate at day 21. EWS/FLI1 and the empty vector serve as controls to measure the capacity of PIM3 to support anchorage-independent growth. EWS/FLI1 is able to promote colony formation, whereas the empty vector does not.