TABLE 2.
Summary of results from the mouse tumorigenic assays
| Cell line | No. with tumor induction/ no. injected | Mean (SE) days to inductiona | % Difference (SE)b | P |
|---|---|---|---|---|
| Expt 1 | ||||
| EWS/FLI1-PIM3 K69M | 4/4 | 32.75 (9) | 46 (21) | 0.04 |
| EWS/FLI1 | 4/4 | 22 (3) | ||
| Tk Neo | 2/4 | 55 (7) | ||
| Expt 2 | ||||
| EWS/FLI1-PIM3 K69M | 8/8 | 20 (1.5) | 38 (6) | 0.001 |
| EWS/FLI1 | 4/4 | 14.5 (1) | ||
| Tk Neo | 3/5 | 48 (3) |
The mean time of tumor formation is the average number of days it took a given cell line to form a 1.5-cm-diameter tumor in a SCID mouse. Data from mice that did not form tumors 80 days postinjection are not included in the average rate of tumor formation.
The average percentage of difference represents the difference in the mean time to tumor formation between the EWS/FLI1 and EWS/FLI1-PIM3K69M cell lines; the empty vector control was not included in this analysis. The percentages of increase in the geometric mean time to tumor formation between the EWS/FLI1 and EWS/FLI1-PIM3 K69M cell lines are shown for both experiments. These increases are significantly different from 0 (P < 0.05 by two-tailed test).