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Snf1 kinase complexes contain Reg1 protein. Snf1 kinase complexes prepared from REG1 and reg1Δ mutant cells were assayed for autophosphorylation activity. Increasing concentrations of Snf1 kinase from the reg1Δ mutant cells were used (lanes 1 to 3). Lanes 2 and 4 contain comparable levels of Snf1 protein (approximately 25 ng/ml).
