FIG. 1.

Rad21 relocates to the centromeres in rec8Δ cells. (A) h⁹⁰ rad21⁺-GFP cells (PY801) carrying pREP81-gar1⁺-CFP during the horsetail period were examined by fluorescence microscopy. In the merged figure, Rad21-GFP and Gar1-CFP are represented by green and red, respectively. The positions of the spindle pole body (SPB), nucleolus, telomere, and centromere are shown in the drawing of the horsetail nucleus. (B) h⁹⁰ rad21⁺-GFP cells of rec8⁺ and rec8Δ (PY801 and PY878) were induced to meiosis and examined for GFP fluorescence during the horsetail period. (C) The localization of Rad21-GFP and Mis6-CFP in h⁹⁰ rad21⁺-GFP rec8Δ cells (PY878) carrying pREP81-mis6⁺-CFP. In the merged figure, Rad21-GFP and Mis6-CFP are represented by green and red, respectively. Bars, 5 μm.