FIG. 3.

Sister chromatid cohesion at the centromere is intact in rec8Δ cells but impaired by the introduction of a rad21-K1 mutation. (A) Chromosomes at the horsetail period are depicted. (B) h⁹⁰ cen1-GFP mei4Δ cells carrying the indicated mutations (wt, PY447; rad21-K1, PY448; rec8Δ, PY446; rec8Δ rad21-K1, PY445) were induced to meiosis at the permissive temperature of 25°C for 8 h and then shifted to 32°C for 4 h. The cells arrested at prophase I (horsetail period) were observed for GFP dots. The number of dots per nucleus is shown with photos of representative nuclei. Bar, 2 μm. (C) Arm cohesion was similarly assayed in wild-type (wt) PY594 and rec8Δ PY595 cells by observing ade3-GFP, which locates in the middle of the left arm of chromosome I.