TABLE 1.

Fission yeast strains used in this study

Strain	Genotype
JY333	h−ade6-M216 leu1
JY450	h90 ade6-M216 leu1
JZ804	h+mei4::ura4+leu1 ura4-D18 ade6-M210
PY182	h90 rec8::kanrade6-M216 leu1
PY221	h+/h+pat1-114/pat1-114 cdc2-L7/cdc2-L7 rec8+-GFP-kanr/rec8+-GFP-kanrade6-M210/ade6-M216 leu2/+ +/lys1
PY322	h+rec8::kanrlys1+<<lacO his7+<<Pdis1-GFP-lac1-NLS leu 1 ade6-M216
PY445	h90 mei4::ura4+rec8::kanrrad21-K1<<ura4+lys1+<<lacO his7+<<Pdis1-GFP-lac1-NLS leu1 ade6-M216
PY446	h90 mei4::ura4+rec8::kanr lys1+<<lacO his7+<<Pdis1-GFP-lac1-NLS leu1 ade6-M216
PY447	h90 mei4::ura4+lys1+<<lacO his7+<<Pdis1-GFP-lacI-NLS leu1 ade6-M216
PY448	h90 mei4::ura4+rad21-K1<<ura4+lys1+<<lacO his7+<<Pdis1-GFP-lac1-NLS leu1 ade6-M216
PY545	h−rec8::kanrleu1
PY594	h90 mei4::ura4+ade3::lacO-ura4+-kanrhis7+<<Pdis1-GFP-lacI-NLS leu1 lys1 ade6
PY595	h90 mei4::ura4+ade3::lacO-ura4+-kanr his7+<<Pdis1-GFP-lacI-NLS leu1 lys1 ade6
PY741	h+/h−mei4::ura4+/mei4::ura4+rec8+-GFP-kanr/rec8+-GFP-kanrade6-M216/ade6-M210
PY748	h−rec8Δ::Padh1-rad21+-FLAG ade6-M210
PY749	h−rec8Δ::Prec8-rad21+-FLAG ade6-M210
PY754	h+/h+pat1-114/pat1-114 cdc2-L7/cdc2-L7 rec8Δ::Padh1-rad21+-GFP/rec8Δ::Padh1-rad21+-GFP leu2/+ +/lys1 ade6-M216/ade6-M210
PY770	h+/h+pat1-114/pat1-114 cdc2-L7/cdc2-L7 rec8Δ::Prec8-rad21+-GFP/rec8Δ::Prec8-rad21+-GFP leu2/+ +/lys1 ade6-M216/ade6-M210
PY783	h−rad21-K1<<ura4+rec8::kanrleu1 ade6-M216
PY785	h+rad21-K1<<ura4+rec8::kanrlys1+<<lacO his7+<<Pdis1-GFP-lacI-NLS ade6-M216 leu1
PY786	h−rad21-K1<<ura4+ade6-M216
PY787	h+rad21+-GFP-kanrmei4::ura4+leu1 ura4-D18 ade6-M210
PY801	h90 rad21+-GFP-kanrleu1 ade6-M210
PY878	h90 rec8::ura4+rad21+-GFP-kanrade6-M216
PY893	h+/h+pat1-114/pat1-114 cdc2-L7/cdc2-L7 rec8::kanr/rec8::kanrrad21+-GFP-kanr/rad21+-GFP-kanrleu2/+ +/lys1 ade6-M216/ade6-M210
PZ207	h90 rec8Δ::Padh1-rec8+-3HA lys1+<<lacO his7+<<Pdis1-GFP-lacI-NLS leu1 ura4-D18 ade6
PZ208	h90 rec8Δ::Padh1-rec8+-3HA rad21::ura4+lysl+<<lacO his7+<<Pdis1-GFP-lac1-NLS leu1 ura4-D18 ade6