Dependence of tP for group 1 events for poly(dA)100 (blue), poly(dC)100 (red), poly(dA50dC50) (orange), poly(dAdC)50 (green), and poly(dCdT)50 (purple). Because all poly(dCdT)50 events fall into one group, that group is considered group 1. All measurements were performed at 120 mV. Bars: SEM of more than five groups of measurements. With rising temperature between 15°C and 40°C, there is a 12-fold decrease of tP1 for the slowest polymer poly(dA) and an 8-fold decrease of tP1 for the fastest poly(dC). The dashed black line that matches closely to the poly(dA50dC50) data is the algebraic average between tP1 of poly(dA)100 and tP1 of poly(dC)100. Note that the temperature dependence is not exponential; rather, ∼T−2 scaling (solid lines) yielded the best fit to the data.