Table 3.
Screening of the Treponema pallidum BAC Genomic Library with Pooled Rabbit Sera
| Clone | Antigen identified | Possible ORFs | Most probable ORF | Function of predicted antigen |
|---|---|---|---|---|
| TP085 | 55 kDa | 0024–0034 | TP0030 | GroELa—heat shock protein 60 (58kDa) |
| TP021 | 68 kDa | 0208–0216 | TP0216 | DnaKb—heat shock protein 70 (68kDa) |
| TP029 | 38 kDa | 0253–0259 | TP0257 | GlpQ—glycerophosphoryldiester phosphodiesterase (41 kDa) |
| 33 kDa | 0304; 0317–0323 | TP0319 | TmpC—membrane lipoprotein (37.8 kDa) | |
| TP201 | 30 kDa | 0860–0864 | TP0862 | peptidyl-prolyl cis-trans isomerase, FKBP-type (28 kDa) |
| TP076 | 36 kDa | 0965 | TP0965 | membrane fusion protein, putative (35 kDa) |
| TP094 | 47 kDa | 0574–0595 | TP0574 | carboxypeptidase (48 kDa) |
| TP003 | 90 kDa | 0429–0437 | TP0435 | lipoprotein, 17 kDa (tpp 17) |
| 55 kDa | 0477–0486 | TP0486 | antigen, p83/100 (53 kDa) | |
| TP173 | 75 kDa | 0747–0755 | TP0748 | CfpA—cytoplasmic filament protein A (79 kDa) |
| 43, 35, 25 | 0768–0773 | TP0768 | TmpA—membrane protein (37 kDa) | |
| kDa | TP0769 | TmpB—outer membrane protein (37 kDa) |
a
GroEL is closely related to heat shock protein homologs from more than 60 different bacteria.
b
Sequence of DnaK proteins are highly conserved among many organisms, e.g., T. pallidum DnaK is more than 58% identical to E. coli DnaK (Bardwell and Craig 1984).