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αz follows γ2MITO to mitochondria. Cells were transfected with γ2MITO, β1, and EE-tagged wild-type αz (a–c) or γ2MITO plus β1 tagged with the EE epitope (d–f). Cells were costained with anti-γ2 (a and d) and anti-EE antibodies (b and e). c and f show the overlap (yellow) of the two antibodies. (Bars = 20 μm.)
