Figure 4.

Effect of removing lipid modification sites on PM and mitochondrial targeting of α_z. Cells were transfected with α_zC3A, which lacks the palmitoylation site (a–d), or with α_zG2AC3A, which lacks both myristoylation and palmitoylation sites (e–h), either alone (d and h) or in combination with γ₂MITO and β₁ (a–c and e–g). Cells were stained with anti-EE antibody (b, d, f, and h; to detect epitope-carrying α_z mutants) and anti-γ₂ antibody (a and e). c and g show the overlap (yellow) of the antibody stains (c shows overlap between a and b; g shows overlap between c and f). (Bar = 20 μm.)