Figure 2.

Expression of G159-defined cyclic sialyl 6-sulfo Lewis X on human leukocytes as ascertained by flow cytometry. (A) Results on peripheral blood leukocytes prepared from a normal individual. (B) Results on representative cultured human leukemic cell lines, YT (an NK-like cell line), ED40515-N (an adult T-cell leukemia cell line derived from helper memory T-cells), and U937 (a monocytic leukemia cell line), and effect of sialidase treatment. (C) Effect of TPA treatment on the expression of the determinant on Jurkat (leukemic cells derived from helper T-cells) and Namalwa (B-cell leukemia) cells. TPA treatment was performed at 20 ng/ml for 3 days for Jurkat cells and 10 ng/ml for 4 days for Namalwa cells.