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. 2002 Jan 1;16(1):101–113. doi: 10.1101/gad.949302

Figure 2.

Figure 2

Figure 2

Figure 2

Figure 2

Ctf3p, Mcm22p, Mcm16p, and Ctf19p coimmunoprecipitate from yeast lysates. The indicated strains were grown to log phase, lysed and anti-Myc immunoprecipitations were performed. Forty micrograms of whole-cell lysate (L) and 25% of total immunoprecipitate (IP) were loaded on the gel. Western blots were performed to detect Myc- or HA-tagged proteins. The yeast strains used were: (A) untagged (No Tag, YVM100), Mcm22p–HA (YVM210), Ctf3p–Myc (YVM218), Mcm22p–HA Ctf3p–Myc (YVM255); (B) untagged (No Tag, YVM100), Mcm22p–Myc (YVM 290), Mcm16p–HA (YVM344), Mcm16p–HA Mcm22p–Myc (YVM346); (C) untagged (No Tag, YVM100), Ctf3p–Myc (YVM218), Mcm16p–HA (YVM344), Ctf3p–Myc Mcm16p–HA (YVM336); and (D) untagged (No Tag, YVM100), Ctf3p–Myc (YVM218), Ctf3p–Myc ctf19Δ (YVM400), Mcm22p–Myc (YVM 290), Mcm22p–Myc ctf19Δ (YVM398), Mcm16p–Myc (YVM325), Mcm16p–Myc ctf19Δ (YVM402). Asterisks are IgG antibody light chain.