Table 3.
Genetic interactions
| Genotype
|
Phenotype
|
|---|---|
| ctf3ΔHIS3 mcm22ΔURA3 mcm16ΔkanMX6 | viable |
| ctf3ΔHIS3 mcm22ΔURA3 mcm16ΔkanMX6 ctf19ΔTRP1 | viable |
| ctf3ΔHIS3 ctf13-30 | CSLa |
| mcm22ΔURA3 ctf13-30 | CSLa |
| mcm16ΔkanMX6 ctf13-30 | CSLa |
| ctf3ΔHIS3 mcm22ΔURA3 mcm16ΔkanMX6 ctf13-30 | CSLa |
| ctf3ΔHIS3 mcm22ΔURA3 mcm16ΔkanMX6 ctf19ΔTRP1 ctf13-30 | CSLa |
| ctf3ΔHIS3 ctf14-42 | SL |
| mcm22ΔURA3 ctf14-42 | SL |
| mcm16ΔkanMX6 ctf14-42 | SL |
| ctf3ΔTRP1 cse4-1 | CSLb |
| ctf19ΔTRP1 cse4-1 | CSLb |
SL, synthetic lethality.
a
CSL, conditional synthetic lethality. ctf3-30 is lethal at 35°C, nonpermissive temperature is reduced to 32°C when combined with ctf3Δ, mcm22Δ, mcm16Δ, ctf3Δmcm22Δmcm16Δ, or ctf3Δmcm22Δmcm16Δctf19Δ mutants.
b
cse4-1 is lethal at 38°C (Stoler et al. 1995), nonpermissive temperature is reduced to 35°C when combined with ctf3Δ or ctf19Δ mutants.