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. 2002 Jan 1;16(1):33–45. doi: 10.1101/gad.949602

Figure 1.

Figure 1

Requirement of Bax in TRAIL-induced apoptosis. (A) Western analysis of Bcl-xl expression in the Bax+/−/Bcl-xl stable cell line and the control Bax+/−/neo cell line. Whole cell extracts were used. Expression of tubulin was used as a control. (B) Suppression of TRAIL-induced apoptosis in Bax+/−/Bcl-xl cells. Cells were treated with TRAIL for 6 h, apoptosis was measured by trypan blue exclusion, and data from three independent experiments were plotted with standard deviations. (C) Western analysis of Bax expression in Bax+/− and Bax−/− cells. (D) Morphology of TRAIL-treated Bax+/− and Bax−/− cells. The picture was taken 6 h after TRAIL treatment. (E) Comparing the expression of GFP–Bax protein in GFP–Bax stable cell lines derived from Bax−/− cells with Bax expression in Bax+/− cells. Cells expressing GFP only were used as a negative control. Whole cell extracts were analyzed. (F) TRAIL-induced apoptosis in Bax−/−, Bax+/−, GFP, and GFP–Bax cells. Cells were treated with TRAIL (100 ng/mL) for 6 h, apoptosis was measured by trypan blue exclusion, and data from three independent experiments were plotted with standard deviations.