Table 1.
Effects of actin- and myosin-modulating drugs on regulated amylase secretion by pancreatic acini
| Drug | Nonstimulated | Stimulated | ||
|---|---|---|---|---|
| Control | 6.6 ± 3.8 (11) | 19.0 ± 5.9 (11) | ||
| Jasplakinolide (3 μM) | 3.0 ± 0.7 (4) | P < 0.05 | 11.1 ± 1.4 (4) | P < 0.05 |
| Cytochalasin D (10 μM) | 7.8 ± 4.0 (8) | NS | 21.7 ± 7.3 (10) | NS |
| Latrunculin B (1 μM) | 7.0 ± 2.7 (6) | NS | 17.7 ± 7.5 (8) | NS |
| BDM (50 mM) | 7.4 ± 3.6 (6) | NS | 5.9 ± 3.3 (6) | P < 0.001 |
| BDM + cytochalasin D | ND | 3.8 ± 1.2 (4) | P < 0.001 | |
| BDM + latrunculin B | ND | 6.1 ± 1.8 (2) | P < 0.01 | |
The effects of various agents that modulate microfilaments and myosin were tested on pancreatic acini that were either nonstimulated or stimulated with carbamylcholine (10 μM). Values, expressed as mean ± SEM, represent the amount of amylase secreted as a percentage of total amylase (sum of amylase released and amylase remaining in acini). Numbers in parentheses indicate how many experiments were performed. Statistical significance of the various drug treatments was calculated with reference to the controls by using Student's t test. ND, not determined; NS, not significant.