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. 2002 Jan 1;16(1):138–149. doi: 10.1101/gad.212702

Figure 4.

Figure 4

Monitoring TT1 expression. (A) TT1 expression detected by RT–PCR in various organs of Ler plants. Ro, roots; yL, young leaves; oL, old leaves; St, stem; Fl, flowers; Si, siliques; Co, no template. The TT1 fragment was detected by ethidium bromide staining after 35 amplification cycles. The ACTIN2 transcript (AC2, 25 cycles) was used as a control. (B,C) Histochemical staining of flowers and siliques of transgenic A. thaliana plants containing the TT1-GUS transgene. Panel B represents flower developmental stage 15, and panel C stage 16 (Smyth et al. 1990). Similar results were obtained with material from several independent transgenic lines. (D,E) Longitudinal sections of siliques of TT1-GUS plants stained for GUS activity. Siliques (stage 15 of flower development) from several independent transgenic lines were analyzed and two representative pictures were selected.