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. 2002 Jan 1;16(1):138–149. doi: 10.1101/gad.212702

Figure 5.

Figure 5

Seed-specific effects of the tt1 mutation. (A) Results of a TLC analysis of flavonoid aglycones accumulating in mature wild-type and tt1-1 mutant seeds. Cyanidin (Cy, red spot) serves as control. (B–D) Vanillin-stained 3-day-old Ler wild-type (B), tt1-1 (C), and tt4 (D) mutant seeds (longitudinal view). The red precipitate indicates presence of catechins. c, chalaza; m, micropyle. (E) BAN expression detected by quantitative RT–PCR after hybridization and autoradiography. Co, no template control; Fb, flower buds; 13, 15 and 16, stages of flower/silique development. Samples were derived from wild-type (Ler) or mutant (tt1-1) plants; the ACTIN2 transcript (AC2) was used as a control. The signals shown were obtained after 17 (BAN) and 13 cycles (ACTIN2). (F–H) Toluidine blue-stained transversal sections of 3-day-old wild-type (F), tt1-1 (G), and tt4 (H) mutant seeds.