Figure 6.

Pattern of PSI expression in Drosophila testes and ovaries. (A) Immunoblot analyses of testes protein extracts prepared from w¹¹¹⁸ males (lane 2) or homozygous v16 males rescued by the PSI (lane 3) or PSIΔAB (lanes 4,5) cDNA transgenes were performed as in Figure 1B. A w¹¹¹⁸ whole-fly protein extract was processed in parallel (lane 1). (B) Testes dissected from homozygous v16 adult males rescued by the PSI (I,III,V) or PSIΔAB#1 (II,IV,VI) cDNA transgenes were fixed and stained with affinity-purified anti-PSI rabbit antibodies and Alexa Fluor secondary antibodies (I,II,V,VI) and with DAPI (III,IV). Magnification of the basal end of a v16;P[PSI] testis (V) or the central part of a v16;P[PSIΔAB#1] testis (VI) shows the nuclear localization of both the PSI and PSIΔAB proteins in somatic and germ-line cells. Scale bar, 50 μm. (C) Stage 4–9 (I), 2–8 (III), 10 (II), or mature (IV) oocytes from w¹¹¹⁸ females were fixed and stained with anti-PSI polyclonal antibodies (I,II) or hybridized with a digoxygenin-labeled anti-sense PSI RNA probe (III,IV). To observe the very low level of PSI protein expression, whole-mount ovarioles were analyzed by confocal imaging. Scale bar, 50 μm.