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. 2006 Aug 10;25(16):3823–3831. doi: 10.1038/sj.emboj.7601264

Figure 7.

Figure 7

Overexpression of 14-3-3σ reduces ARE-mRNA half-life. CHO-14-3-3σ cells were treated with Dox for 3 days or mock treated, then cotransfected with plasmids expressing the LacZ-ARE or stable LacZ reporter and GFP mRNAs. Cells were treated with Act D for up to 4 h. Total RNA was extracted and analyzed by Northern blot analysis using β-gal and GFP probes. A representative Northern blot from three independent experiments is shown. mRNA levels were quantified by densitometry and mRNA half-lives (t1/2) determined from a log10 semilog plot, normalized to GFP and the initial levels of the β-gal mRNA.