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. 2006 Aug 10;25(16):3750–3761. doi: 10.1038/sj.emboj.7601274

Figure 4.

Figure 4

Effects of rab depletion on OCRL1 targeting. (A) HeLa cells were transfected with control siRNA (luciferase) or siRNAs targeting rab1A alone, rab6 alone, or rab1A and rab6 together for 72 h and analysed by Western blotting with the indicated antibodies. (B) SiRNA treated cells were analysed by immunofluorescence microscopy with antibodies to OCRL1 and either GalNacT2 or rab6 as indicated. Nontransfected cells are marked with an asterisk. Depletion of rab1A is indicated by Golgi fragmentation. (C) SiRNA-treated cells were analysed by immunofluorescence microscopy following triple staining with antibodies to OCRL1, rab6, and GalNacT2. Nontransfected cells are marked with an asterisk. Bar, 10 μm.