Membrane recruitment of OCRL1 is required for regulation of Golgi and endosomal dynamics. HeLa cells expressing GFP OCRL1Δ237–539 or GFP OCRL1Δ237–539/S564P were analysed by immunofluorescence microscopy with antibodies to GalNacT2 (A), transferrin receptor (TfR) (B), or the cation-independent mannose 6-phosphate receptor (CI-MPR) (C). Bar, 10 μm. (D) Quantitation of the Golgi, CI-MPR, and TrR phenotypes. The results are expressed as the mean±s.d. from three independent experiments, counting 100 cells per experiment.