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Rabs 5 and 6 stimulate OCRL1 5-phosphatase activity. (A) Incubations were performed in the absence or presence of full-length, WT OCRL1 with or without 2.4 μM GST-tagged rab5Q79L, rab6Q72L, or rac1Q61L as indicated. (B) WT OCRL1 or the G664D mutant were incubated with rab6Q72L as indicated. PtdIns(4,5)P2 5-phosphatase activity was measured as described in the Materials and methods. Results are expressed as the mean±s.d. of three independent experiments.
