Figure 5.

uPAR and β-catenin expression levels are increased concomitantly in primary carcinomas and their liver metastases. (a) Total RNA (25 μg per lane) from tissue samples of normal colonic mucosa (N), primary tumor (T), and metastases (M) were hybridized with probes for uPAR, β-catenin, or β-actin. (b) Immunoblot of whole cell lysates, developed with anti-uPAR rabbit polyclonal antibody 399R or with anti-β-actin mAb 5441. Representative blots from three of six analyzed patients are shown in a and b. (c) Expression of β-catenin mRNA, uPAR mRNA, and uPAR protein in tissue samples from N, T, and M of six analyzed patients after normalization to β-actin expression on the same blots. Bars indicate two standard deviations.