Figure 2.

Location analysis of E2F4 on human promoters and identification of previously known E2F target genes. (A) Scatter plot analysis of Cy3-labeled total genomic DNA versus Cy5-labeled, E2F4 ChIP-enriched DNA. A P-value cutoff of 0.01 is shown. (B) Confirmation of promoter occupancy by E2Fs in quiescent WI-38 cells using a standard ChIPs protocol. E2Fs were enriched at several promoters involved in cell cycle control and DNA replication. In contrast, we did not detect enrichment of a negative control β-interferon fragment with anti-E2F4 antibodies. Nor did we observe significant promoter enrichment with an irrelevant antibody control (mock) lanes. Input lanes correspond to PCR reactions with 0.5% of total chromatin in immunoprecipitation reactions.