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. 2006 Aug 15;20(16):2279–2292. doi: 10.1101/gad.384506

Figure 5.

Figure 5.

MPK-1 and GLA-3 interact in vitro and in vivo. (A) Myc-GLA-3 and GST-MPK-1 interact in 293T cells. 293T cells were transfected with plasmids expressing Myc-tagged GLA-3A or GLA-3B isoforms and GST-MPK-1A or GST-MPK-1B. GSTMPK-1A or GST-MPK-1B was affinity-purified using glutathione beads, and the presence of Myc-GLA-3A or Myc-GLA-3B was detected by immunoblotting using an anti-Myc antibody. (B) MPK-1 interacts with GLA-3 in C. elegans. Total protein was extracted from synchronized adult hermaphrodites, and immunoprecipitation was performed using anti-GLA-3A or GLA-3B antibodies. Pre-immune serum was used as a control. Immunoprecipitated samples were probed with an antibody against mammalian ERK1/2, which recognizes C. elegans MPK-1 (top panel) (Lackner and Kim 1998) and with antibodies against GLA-3A or GLA-3B (bottom panel).