Figure 1.

Known flagellar T3S-chaperones do not facilitate FlgM secretion. A graph showing the relative amount of intracellular FlgM (FlgM_IN—unshaded bars) compared with extracellular FlgM levels (FlgM_OUT—black bars). Secretion assays were performed as described in Materials and Methods. FlgM_IN is defined as the level of FlgM present in whole cell lysates of mid-log phase cultures, while FlgM_OUT is defined as the amount of FlgM detected in the supernatant of the same culture. The secretion assays were performed in a wild-type background (HBB⁺ fliC ⁺) using null mutants of all three known T3S-chaperones compared with the wild-type strain LT2 (TH437): ΔflgN = TH5937; ΔfliS = TH5737; ΔfliT = TH5831; ΔfliS ΔfliT = TH5935; ΔfliS ΔfliT ΔflgN = TH5999. All data, including error bars, are shown relative to LT2 FlgM_IN levels. The data shown are an average of three independent repeats of the secretion assays.