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. 2002 Jan 15;16(2):198–208. doi: 10.1101/gad.950302

Figure 3.

Figure 3

Phosphorylation of Rad17 by ATR and ATM. (A) HCT116 (ATR+/+) or its ATRflox/− derivative cells infected with adenovirus expressing Cre (Ad-Cre) or adenovirus expressing GFP (Ad-GFP) were collected at the indicated times after infection. Extracts were prepared from these cells and analyzed by immunoblotting with antibodies to ATR and ATM. (B) ATR+/+ and ATRflox/− cells were infected with Ad-Cre or Ad-GFP as indicated. Four days after infection, the cells were treated with 50 J/m2 of UV, 20 Gy of γ-IR, or 1 mM of HU and collected at the indicated times after the treatments. Extracts of the cells were analyzed by immunoblotting with the indicated antibodies. (C) A-T cells (AT22IJE-T) or A-T cells complemented with ATM (AT22IJE-T/YZ5) were treated with 20 Gy of γ-IR and collected at the indicated times after irradiation. Extracts of the cells were analyzed by immunoblotting with the indicated antibodies. (*) A protein nonspecifically recognized by the anti-ATM antibody.