Figure 1.

Colocalization of Shi and Lqf proteins in eye discs. (A–J) Apical views of a third instar larval eye disc, double-labeled with anti-Shi and anti-Lqf. (A–C) Lqf and Shi colocalize in cells within and posterior to the morphogenetic furrow, indicated by the arrow in A. (D–F) An enlargement of the area near the furrow shows that Lqf and Shi are concentrated at the apical tips of cells within the furrow. (G–I) An enlarged view of the area posterior to the furrow shows that Lqf and Shi are concentrated in dots, which are the apical membranes of the photoreceptors (R-cells), where they meet (see below). In addition, Lqf and Shi colocalize in a lattice, which is made up of the membranes of the surrounding cells. (J) A further enlargement of G, showing Lqf membrane localization in four adjacent facets. (K,L) An apical view of four adjacent facets in eye discs double-labeled with anti-Lqf and anti-Elav (K), which labels R-cell nuclei, or anti-Lqf and anti-Cut (L), which labels cone cell nuclei. There is no overlap in the localization of Lqf protein and either Elav or Cut; Lqf is outside the nuclei (which fill the apical cytoplasm) and in the central region of the developing facet where the photoreceptor cell membranes meet.