Figure 2.

Detection of Lqf and Shi proteins in faf⁻ clones in eye disc. Apical views of two different third instar larval eye discs are shown in A–D and E–H. (A,E) The faf⁻ clones are labeled by the absence of β-gal protein. (B,F) The clone shapes are apparent as areas with lower levels of Lqf protein. (C,G) The clone shapes in A and E were outlined in white and layered over the panels in B and F. (D,H) The levels of Shi protein are unaffected in the faf⁻ clones. We know that detection of the Lqf protein signal is unaffected by the β-gal protein signal, as clones are visible as areas of lower levels of Lqf signal in discs labeled only with anti-Lqf. A few of the clone areas in A and E are not obviously mirrored in B and F. This is because of the subtlety of the Lqf concentration difference (<twofold) often being detected; although there is only two- to threefold less Lqf in faf⁻/faf⁻ eye discs than in wild-type (faf⁺/faf⁺), the clones are faf⁻/faf⁻, but the surrounding cells are often faf⁻/faf⁺ (the clone twin spots are faf⁺/faf⁺). Slight variability in antibody penetration and so on within the disc can affect the staining and obscure concentration differences in parts of the disc. Nevertheless, it is clear that the clone shapes are generally present in the Lqf-stained discs (B,F), but not in the Shi-stained discs (D,H).