Figure 3.

Comparison of protein levels in wild-type and faf⁻ eye discs. (A) A histogram showing the level of Lqf protein, normalized to tubulin, in wild-type and faf⁻ eye discs. The wild-type value was arbitrarily set to 1.0. Examples of Western blots used to generate this data are shown in Figure 4A below. (B) A histogram showing the level of Lqf protein, normalized to tubulin, in faf^BX4 eye discs, and in faf^BX4 eye discs with a copy of either a faf⁺ transgene or a faf⁻ transgene. The faf^BX4 value was arbitrarily set to 1.0. An example of a Western blot used to generate these data is shown in Figure 4B below. (C) A histogram showing the levels of four different endocytosis complex proteins and Armadillo (Arm), normalized to tubulin, in wild-type (wt; black bars) and faf^BX4 (gray bars) eye discs. The wild-type value was arbitrarily set to 1.0. Standard errors in A–C were calculated from differences in repeated experiments.