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. 2002 Feb 1;16(3):301–306. doi: 10.1101/gad.959102

Figure 5.

Figure 5

Gfi-1b−/− fetal livers show arrested megakaryopoiesis. (A) Colonies (a,d) and cells (b,c,e,f) from control (a–c) and Gfi-1b−/− (d–f) fetal liver cells grown in thrombopoietin (tpo). When cultured in tpo, fetal liver cells from wild-type livers give colonies with large megakaryocytes (a), whereas Gfi-1b−/− cells proliferate in tpo but do not differentiate into large megakaryocytes (d). The Gfi-1b−/− cells also do not show any nuclear (multilobulation) or cytoplasmic (granulation) maturation upon May–Grunwald–Giemsa staining (b vs. e) and are negative for acetylcholine esterase staining (c vs. f). (B) Semiquantitative RT–PCR of control (wild-type) and Gfi-1b−/− fetal liver cells cultured in tpo. Gfi-1b−/− cells have far fewer transcripts encoding markers of mature megakaryocytes relative to controls (a–d, lanes 5–8 vs. 1–4), for example, von-Willebrand factor (vWF in a), the transcription factor NF-E2 (b), the c-MPL receptor (c), and the surface glycoprotein IIb (d).