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. 2002 Feb 1;16(3):388–398. doi: 10.1101/gad.955202

Figure 6.

Figure 6

UPD protein binds MOM. Transfected 293T cells were incubated with concentrated conditioned medium from 293T cells, producing UPD–V5. Following incubation with conditioned medium, the cells were washed, fixed in paraformaldehyde/PBS, and incubated with mouse anti-V5 and rabbit anti-STAT92E antibodies. Only 293T cells expressing STAT92E/HA–MOM and STAT92E/HA–MOM-N show strong staining when incubated with UPD and anti-V5 antibody (left and middle panels). Cells transfected with HA–STAT92E alone show a background staining when incubated with UPD-containing conditioned medium (right panel). These results suggest that UPD specifically binds to 293T cells expressing MOM.