Figure 1.

Outline of lin-35 synthetic screen. Following chemical mutagenesis, three consecutive generations of kuEx119⁺ animals are single-cloned and allowed to self-fertilize. Animals acquiring silent or otherwise viable mutations (m) that show synergy with lin-35 are identified by the absence of wild-type-appearing kuEx119⁻ progeny in the final (F₃) generation. Only animals carrying mutations are shown in subsequent generations. For further details, see text.