Table 1.

Oligonucleotides used in this study

Name	Sequence*	Purpose
Ex-1b-f	GCGAATTCCAGTAGCCAAGGACTAGTAG	Forward and reverse primers to make exon 1b fragment
Int-1b-r	GCGGATCCAGAATTGCTCGCGCCCTTAG
Int-1b-f	GCGGATCCAGTGAATGTGCCGCTGCAGT	Forward and reverse primers to make exon 4 fragment
Int-4-r	GCGGTACCTCGGAGAGGGAACTGTAATC
Int-4-s	GCGGTACCTGGCTGAGTGATCAAACCGT	Forward and reverse primers to make exon 5 fragment
Ex-5-r	CGAAGCTTGGCGGCGCGTAAGGACAGG
IVS4+5G>C-f	GAGTCCGGGTAGcAGCCAGCACGGAG	Forward and reverse overlap PCR primers to make IVS4+5G>C mutant
IVS4+5G>C-r	CTCCGTGCTGGCTgCTACCCGGACTC
IVS5-11A>G-f	CTCCCTTGCCCCAgCCGCCCCCAGG	Forward and reverse overlap PCR primers to make IVS5-11A>G mutant
IVS5-11A>G-f	CCTGGGGGCGGcTGGGGCAAGGGAG
IVS4-1G>T-f	CGTTTTCAtAGAAAGAT	Forward and reverse overlap PCR primers to make IVS4-1G>T mutant
IVS4-1G>T-f	ATCTTTCTaTGAAAACG
T7 promoter	TAATACGACTCACTATAGGG	Forward primer to pcDNA T7 promoter to detect minigene mRNA
PCREx-1b-f	TGTCGGCCGTCTCCTCATCTTCC	Forward and reverse primers to detect endogenous and minigene mRNA
PCREx-5-r	TTGCGCTCCCTCTTTCTCCATTTG
GFP-f	GACGGCAACATCCTGGGGCACAAG	Forward and reverse primers to GFP
GFP-r	CGGCGGCGGTCACGAACTCC
GAPDH-f	TGATGACATCAAGAAGGTGGTGAAG	Forward and reverse primers to GAPDH
GAPDH-r	TCCTTGGAGGCCATGTGGGCCAT

*Primers are 5' to 3', mutations are in lowercase