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. 2006 Jun 7;5:22. doi: 10.1186/1476-4598-5-22

Figure 6.

Figure 6

Verification of gene array results using RT-PCR. Shown are ethidium bromide-stained PCR fragments of six different genes found to be up-regulated 10-fold or more in CDDO-Im-treated iMycEμ-1 and-2 cells. The iMycEμ-1 and-2 cells had been treated for 24 hrs with 0.4 μM and 1 μM CDDO-Im, respectively. The fragments were compared to those detected in untreated cells using the same assay conditions. See Table 1 for further details and Additional File 2 for PCR primers and reaction conditions.