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. 2002 Mar 1;16(5):608–619. doi: 10.1101/gad.967602

Figure 1.

Figure 1

Crz1p nuclear export is regulated by calcineurin. nmd5Δ (HFY133) cells expressing 3xGFP–SV40NLS (LMB134), 3xGFP–SV40NLS–Crz1p (LMB148), or 3xGFP–Crz1p (LMB127) were grown to log phase at 21°C. Cells were treated with 200 mM CaCl2 or pretreated with FK506 (2 μg/mL) for 30 min before CaCl2 addition and visualized using fluorescence microscopy. Bar, 20 μm.