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. 2002 Mar 1;16(5):608–619. doi: 10.1101/gad.967602

Figure 3.

Figure 3

Msn5p is required for Crz1p nuclear export. WT (ASY472) and msn5Δ (ASY788) cells expressing GFP–Crz1p (AMS463) were grown to log phase at 21°C. Cells were treated with cycloheximide (10 μg/mL) for 20 min. CaCl2 (200 mM) was added for 10 min followed by FK506 (5 μg/mL). Images were captured 30 min after FK506 treatment. Bar, 20 μm.