Table 2.
Plasmids used in this study
| Plasmid
|
Description
|
Reference
|
|---|---|---|
| AMS367 | URA3-4xCDRE integrating plasmid | Stathopoulos and Cyert 1997 |
| AMS446 | HA-CRZ1 | Stathopoulos and Cyert 1997 |
| AMS463 | GFP-CRZ1 | Stathopoulos-Gerontides et al. 1999 |
| pVT CNA2 | CNA2 trunc | Withee et al. 1997 |
| pOM4 | 3xGFP vector | Polizotto and Cyert 2001 |
| LMB127 | 3xGFP-CRZ1 | This study |
| LMB134 | 3xGFP-SV40NLS vector | This study |
| LMB148 | CRZ1 in LMB134 | This study |
| LMB158 | CRZ1 (aa 186–422) in LMB134 | This study |
| LMB160 | CRZ1 (aa 186–340) in LMB134 | This study |
| LMB162 | CRZ1 (aa 186–279) in LMB134 | This study |
| LMB163 | CRZ1 (aa 186–237) in LMB134 | This study |
| LMB165 | CRZ1 (aa 433–679) in LMB134 | This study |
| LMB178 | CRZ1 (aa 1–186) in LMB134 | This study |
| LMB180 | CRZ1 (aa 186–310) in LMB134 | This study |
| LMB184 | CRZ1 (aa Δ186–233) in LMB 134 | This study |
| LMB186 | CRZ1 (mSRR) in LMB 134 | This study |
| LMB199 | CRZ1 (aa 201–279) in LMB134 | This study |
| LMB207 | CRZ1 (aa 186–250) in LMB134 | This study |
| LMB211 | mSRR (aa 186–279) in LMB134 | This study |
| LMB228 | PIISIQΔ (aa 186–340) in LMB134 | This study |
| LMB189 | GAL4AD-CRZ1 (aa 186–340) | This study |
| LMB193 | GAL4AD-CRZ1 (aa 186–279) | This study |
| LMB218 | GAL4AD-mSRR (aa 186–279) | This study |
| LMB224 | GAL4AD-PIISIQΔ (aa 186–340) | This study |
| LMB226 | GAL4AD-CRZ1 (aa 186–310) | This study |
| LMB230 | CRZ1 (aa Δ311–340) in pOM4 | This study |
| LMB231 | CRZ1 (aa Δ331–336) in pOM4 | This study |
| BM3694 | GAL4BD-MSN5 | DeVit and Johnston 1999 |
| BJP2014 | GAL4BD-CNA1 | Jiang and Cyert 1999 |