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. 2002 Mar 1;16(5):620–632. doi: 10.1101/gad.955702

Figure 5.

Figure 5

Antibodies to GEX-2 and GEX-3 show localization to the boundaries of all cells starting at the two-cell stage. GEX-2 has a distinct localization pattern compared with AJM-1. Wild-type embryos are shown with dorsal up and anterior to the left. (a,b) Antibodies to GEX-3 on embryos at the 8-cell (a) and at the early comma stage (b) show GEX-3 expression in the hypodermis as well as at the boundaries of other cells. (c–f) Antibodies to GEX-2 on embryos at the 32-cell (c), 1.5-fold stage (d), and at the late comma stage (e,f) show GEX-2 is found at cell boundaries in the hypodermis, which has enclosed the embryo and is beginning to constrict to squeeze the embryo into a worm (d,e). (g,h) Antibodies to AJM-1 reveal that GEX-2 does not colocalize with the AJM-1 junctional domain. (i,j) Merged image. The focal plane in e–f is though the center of the embryo. f, h, and j are close-ups of a dorsal section of the images in e, g, and i.