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. 2002 Mar 1;16(5):571–582. doi: 10.1101/gad.970702

Figure 8.

Figure 8

The ATM/NBS1/SMC1 branch of the S-phase checkpoint. (a) Kinetics of degradation and accumulation of CDC25A in response to 10 Gy of IR in NBS cells that were complemented with the vector, wild-type, and S278A/S343A NBS1 cDNA. (b) CHK2 activation in response to 10 Gy of IR and 1 h of recovery in NBS cells complemented with the vector, wild-type, and S278A/S343A NBS1 cDNA and A-T cells complemented with the vector and wild-type ATM cDNA. CHK2 was detected by Western blotting. The slowly migrating form marked with an arrow is labeled with Pi-CHK2. (c) A simplified S-phase checkpoint pathway in response to DSBs in mammalian cells. Phosphorylated NBS1 (Pi-NBS1) is depicted as an adaptor.