Figure 5.

AIR-2 kinase is required for localization of condensin proteins during mitosis. (A–D) Wild-type embryo costained with SMC-4 antibody (green, A), DNA dye (red, B), and tubulin antibody (blue, C; merge in D). SMC-4 associates with condensed mitotic chromosomes within mitotic spindles, but not with interphase chromosomes. (Arrowhead) Mitotic nucleus; (arrow) interphase nucleus. (E–H) air-2(RNAi) embryo, stained as above. SMC-4 (E) does not associate with condensed mitotic chromosomes (F) within a tetrapolar mitotic spindle (G; merge in H). (I–L) air-2(RNAi) embryo, costained with HCP-3 antibody (green, I), DNA dye (red, J), and tubulin antibody (blue, K; merge in L). HCP-3 associates with mitotic chromosomes when AIR-2 is depleted.