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. 2003 Jun;185(11):3325–3332. doi: 10.1128/JB.185.11.3325-3332.2003

FIG. 3.

FIG. 3.

Evidence of a covalently linked 5′ TP. (A) DNA-protein complex (lane 1), complex treated with exonuclease III for 15 min (lane 2), complex treated with exonuclease III for 30 min (lane 3), PK-DNA (lane 4), PK-DNA treated with exonuclease III for 15 min (lane 5), and PK-DNA treated with exonuclease III for 30 min (lane 6). (B) DNA-protein complex (lane 1), complex treated with λ exonuclease for 15 min (lane 2), complex treated with λ exonuclease for 30 min (lane 3), PK-DNA (lane 4), PK-DNA treated with λ exonuclease for 30 min (lane 5), PK-DNA treated with 0.5 M piperidine (lane 6), PK-DNA treated with piperidine and λ exonuclease for 30 min (lane 7). The amounts used are indicated in Materials and Methods.