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. 1972 May;11(1):89–98.

Activities of immunosuppressive agents in vitro. II. Different timing of azathioprine and methotrexate in inhibition and stimulation of mixed lymphocyte reaction

Marie-Anne Bach, J-F Bach
PMCID: PMC1553678  PMID: 5038773

Abstract

Azathioprine (AZ) and Methotrexate (MTX) inhibit thymidine incorporation in mixed lymphocyte reaction (MLR) at non-cytotoxic concentrations. AZ is active only when introduced in the culture during the first 24 hr, whereas MTX is still active when introduced at day 4. These data indicate that AZ might act on antigen recognition by T cells, as previously suggested by its effects on inhibition of rosette formation, and not on cell proliferation, as MTX probably does. Stimulation of thymidine incorporation has been observed at low drug concentrations, especially when the drugs were added more than 24 hr after the beginning of the culture, and most particularly for MTX added 6 days after start of the culture.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERENBAUM M. C. The effect of cytotoxic agents on the production of antibody to T.A.B. vaccine in the mouse. Biochem Pharmacol. 1962 Jan;11:29–44. doi: 10.1016/0006-2952(62)90088-6. [DOI] [PubMed] [Google Scholar]
  2. Bach F. H., Voynow N. K. One-way stimulation in mixed leukocyte cultures. Science. 1966 Jul 29;153(3735):545–547. doi: 10.1126/science.153.3735.545. [DOI] [PubMed] [Google Scholar]
  3. Bach J. F., Dardenne M. Activities of immunosuppressive agents in vitro. I. Rosette inhibition by azathioprine. Rev Eur Etud Clin Biol. 1971 Oct;16(8):770–777. [PubMed] [Google Scholar]
  4. Bach J. F., Dardenne M. Etudes sur le métabolisme de l'azathioprine. C R Acad Sci Hebd Seances Acad Sci D. 1970 Jul 27;271(4):453–456. [PubMed] [Google Scholar]
  5. Bach J. F., Dardenne M., Fournier C. In vitro evaluation of immunosuppressive drugs. Nature. 1969 Jun 7;222(5197):998–999. doi: 10.1038/222998a0. [DOI] [PubMed] [Google Scholar]
  6. Bach J. F., Debray-Sachs M., Crosnier J., Kreis H., Dormont J. Correlation between mixed lymphocyte culture performed before renal transplantation and kidney function. Clin Exp Immunol. 1970 Jun;6(6):821–827. [PMC free article] [PubMed] [Google Scholar]
  7. Bach J. F., Muller J. Y., Dardenne M. In vivo specific antigen recognition by rosette forming cells. Nature. 1970 Sep 19;227(5264):1251–1252. doi: 10.1038/2271251a0. [DOI] [PubMed] [Google Scholar]
  8. Bain B. Tritiated-thymidine uptake in mixed leucocyte cultures: effect of specific activity and exposure time. Clin Exp Immunol. 1970 Feb;6(2):255–262. [PMC free article] [PubMed] [Google Scholar]
  9. Denman E. J., Denman A. M., Greenwood B. M., Gall D., Heath R. B. Failure of cytotoxic drugs to suppress immune responses of patients with rheumatoid arthritis. Ann Rheum Dis. 1970 May;29(3):220–231. doi: 10.1136/ard.29.3.220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Elion G. B. Symposium on immunosuppressive drugs. Biochemistry and pharmacology of purine analogues. Fed Proc. 1967 May-Jun;26(3):898–904. [PubMed] [Google Scholar]
  11. Harris R., Ukaejiofo E. O. Rapid preparation of lymphocytes for tissue-typing. Lancet. 1969 Aug 9;2(7615):327–327. doi: 10.1016/s0140-6736(69)90096-8. [DOI] [PubMed] [Google Scholar]
  12. Laborde A., Bach J. F. Inhibition et stimulation par l'azathioprine de l'incorporation de thymidine par les lymphocytes en culture mixte. C R Acad Sci Hebd Seances Acad Sci D. 1971 Jan 18;272(3):509–512. [PubMed] [Google Scholar]
  13. Quadracci L. J., Hellström I. E., Striker G. E., Marchioro T. L., Hellström K. E. Short communication immune mechanisms in human recipients of renal allografts. Cell Immunol. 1970 Nov;1(5):561–566. doi: 10.1016/0008-8749(70)90042-0. [DOI] [PubMed] [Google Scholar]
  14. Rowley M. J., Mackay I. R., McKenzie I. F. Antibody production in immunosuppressed recipients of renal allografts. Lancet. 1969 Oct 4;2(7623):708–710. doi: 10.1016/s0140-6736(69)90425-5. [DOI] [PubMed] [Google Scholar]
  15. Takiguchi T., Adler W. H., Smith R. T. Cellular recognition in vitro by mouse lymphocytes. Effects of neonatal thymectomy and thymus graft restoration on alloantigen and PHA stimulation of whole and gradient-separated subpopulations of spleen cells. J Exp Med. 1971 Jan 1;133(1):63–80. doi: 10.1084/jem.133.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wigzell H. Use of humoral or cellbound antibody to achieve depression of immunological reactivity. Antibiot Chemother. 1969;15:82–97. doi: 10.1159/000386773. [DOI] [PubMed] [Google Scholar]

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