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Localization of Pho84p-GFP is regulated in response to extracellular phosphate levels. Wild type (EY0664, Upper) and an end4ts mutant (EY0687, Lower) harboring pPH084-GFP (EB0666) were studied. Direct fluorescence microscopy of Pho84p-GFP was performed in no-phosphate medium at 25°C (Left) and then in medium containing phosphate (final concentration 10 mM) and cycloheximide (final concentration 0.1 mg/ml) at 33°C.
