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Pho86p-GFP is localized to the ER. Cells lacking PHO86 (EY0665) carrying pPHO86-GFP (EB0667) were starved for phosphate. Direct fluorescence of Pho86p-GFP was observed. Indirect immunofluorescence was performed by using an antibody to the ER-resident protein Kar2p/Bip. From left to right are fluorescence images corresponding to Pho86p-GFP (green), Kar2p (red), or an overlay of the two.
