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Pho84p-GFP is localized to the ER in the absence of PHO86. A pho86Δ strain with integrated PHO84-GFP (EY0669) was starved for phosphate and coimmunofluorescence was performed as in Fig. 2. From left to right are fluorescence images corresponding to Pho84p-GFP (green), Kar2p (red), or an overlay of the two.
