TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| E. coli TOP10 | Cloning strain | Invitrogen |
| E. coli S17-1 | Conjugal transfer of plasmids | 32 |
| C. crescentus CB15N | Synchronizable wild-type strain | Laboratory collection |
| A. tumefaciens C58 | Wild-type strain | Laboratory collection |
| S. meliloti 1021 | Wild-type strain | Laboratory collection |
| LS2305 | C. crescentus CB15N with pMR10 | Laboratory collection |
| LS3607 | A. tumefaciens C58 with pMR10 | This study |
| LS3592 | S. meliloti 1021 with pMR10 | This study |
| Plasmids | ||
| pMR10 | Kan derivative of RK2; broad-host-range low-copy-number vector | R. Roberts and C. Mohr |
| pCR-XL-TOPO | Kan vector for cloning PCR products | Invitrogen |
| Derivatives of pCR-XL-TOPO (Invitrogen) containing FISH probes | ||
| pLK317 | A. tumefaciens circular chromosome origin probe | This study |
| pLK322 | A. tumefaciens linear chromosome repABC probe 1 | This study |
| pLK323 | A. tumefaciens linear chromosome repABC probe 2 | This study |
| pLK339 | A. tumefaciens pAtC58 repABC probe 1 | This study |
| pLK340 | A. tumefaciens pAtC58 repABC probe 2 | This study |
| pLK341 | A. tumefaciens pTiC58 repABC probe 1 | This study |
| pLK342 | A. tumefaciens pTiC58 repABC probe 2 | This study |
| pLK333 | S. meliloti circular chromosome origin probe 1 | This study |
| pLK334 | S. meliloti circular chromosome origin probe 2 | This study |
| pLK335 | S. meliloti pSymA repABC probe 1 | This study |
| pLK336 | S. meliloti pSymA repABC probe 2 | This study |
| pLK337 | S. meliloti pSymB repABC probe 1 | This study |
| pLK338 | S. meliloti pSymB repABC probe 2 | This study |