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. 1973 Apr;13(4):487–496.

Quantitation of surface-membrane and intracellular gamma, mu and kappa chains of normal and neoplastic human lymphocytes

A G Cooper, M C Brown, H A Derby, H H Wortis
PMCID: PMC1553745  PMID: 4717093

Abstract

Using automated haemagglutination and haemagglutination-inhibition methods which are capable of measuring gamma, mu or kappa chains in the nanogram range, we have examined a variety of normal and neoplastic human lymphocyte populations for the amounts of their surface-membrane and internal immunoglobulins. We find IgG to be the predominant immunoglobulin on the surface-membrane and within various normal human lymphocytes, including thymocytes. IgM, in addition to IgG, is found on the surface of tonsil cells. Chronic lymphatic leukaemic lymphocytes consistently had small amounts of surface-membrane immunoglobulin and larger amounts internally (usually IgM), whereas large amounts of immunoglobulin (usually IgM) were present on the surface-membranes and internally in leukaemic lymphosarcoma cells. Both types of cells had the B-specific surface-antigens. Different types of lymphatic leukaemias may represent neoplastic counterparts of different stages of B cell differentiation.

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Selected References

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  1. Ada G. L. Antigen binding cells in tolerance and immunity. Transplant Rev. 1970;5:105–129. doi: 10.1111/j.1600-065x.1970.tb00358.x. [DOI] [PubMed] [Google Scholar]
  2. Basten A., Miller J. F., Warner N. L., Pye J. Specific inactivation of thymus-derived (T) and non-thymus-derived (B) lymphocytes by 125I-labelled antigen. Nat New Biol. 1971 May 26;231(21):104–106. doi: 10.1038/newbio231104a0. [DOI] [PubMed] [Google Scholar]
  3. Coombs R. R., Feinstein A., Wilson A. B. Immunoglobulin determinants on the surface of human lymphocytes. Lancet. 1969 Nov 29;2(7631):1157–1160. doi: 10.1016/s0140-6736(69)92484-2. [DOI] [PubMed] [Google Scholar]
  4. Cooper A. G. Purification of cold agglutinins from patients with chronic cold haemagglutinin disease. Evidence of their homogeneity from starch gel electrophoresis of isolated light chains. Clin Exp Immunol. 1968 Sep;3(7):691–702. [PMC free article] [PubMed] [Google Scholar]
  5. Cooper M. D., Lawton A. R., Bockman D. E. Agammaglobulinaemia with B lymphocytes. Specific defect of plasma-cell differentiation. Lancet. 1971 Oct 9;2(7728):791–794. doi: 10.1016/s0140-6736(71)92742-5. [DOI] [PubMed] [Google Scholar]
  6. Fröland S., Natvig J. B., Berdal P. Surface-bound immunoglobulin as a marker of B lymphocytes in man. Nat New Biol. 1971 Dec 22;234(51):251–252. doi: 10.1038/newbio234251a0. [DOI] [PubMed] [Google Scholar]
  7. Gonatas N. K., Antoine J. C., Stieber A., Avrameas S. Surface immunoglobulins of thymus and lymph node cells demonstrated by the peroxidase coupling technique. Lab Invest. 1972 Mar;26(3):253–261. [PubMed] [Google Scholar]
  8. Greaves M. F., Torrigiani G., Roitt I. M. Inhibition of human mixed lymphocyte reaction by antibodies to immunoglobulin light chain determinants. Clin Exp Immunol. 1971 Sep;9(3):313–328. [PMC free article] [PubMed] [Google Scholar]
  9. Grey H. M., Rabellino E., Pirofsky B. Immunoglobulins on the surface of lymphocytes. IV. Distribution in hypogammaglobulinemia, cellular immune deficiency, and chronic lymphatic leukemia. J Clin Invest. 1971 Nov;50(11):2368–2375. doi: 10.1172/JCI106735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hurez D., Flandrin G., Preud'homme J. L., Seligmann M. Unreleased intracellular monoclonal macroglobulin in chronic lymphocytic leukaemia. Clin Exp Immunol. 1972 Feb;10(2):223–234. [PMC free article] [PubMed] [Google Scholar]
  11. Hämmerling U., Rajewsky K. Evidence for surface-associated immunoglobulin on T and B lymphocytes. Eur J Immunol. 1971 Dec;1(6):447–452. doi: 10.1002/eji.1830010608. [DOI] [PubMed] [Google Scholar]
  12. LEVINE S. Magnetic techniques for in vitro isolation of leucocytes. Science. 1956 Feb 3;123(3188):185–186. doi: 10.1126/science.123.3188.185-a. [DOI] [PubMed] [Google Scholar]
  13. Lerner R. A., McConahey P. J., Dixon F. J. Quantitative aspects of plasma membrane-associated immunoglobulin in clones of diploid human lymphocytes. Science. 1971 Jul 2;173(3991):60–62. doi: 10.1126/science.173.3991.60. [DOI] [PubMed] [Google Scholar]
  14. Lesley J. F., Kettman J. R., Dutton R. W. Immunoglobulins on the surface of thymus-derived cells engaged in the initiation of a humoral immune response. J Exp Med. 1971 Sep 1;134(3 Pt 1):618–629. doi: 10.1084/jem.134.3.618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Marchalonis J. J., Atwell J. L., Cone R. E. Isolation of surface immunoglobulin from lymphocytes from human and murine thymus. Nat New Biol. 1972 Feb 23;235(60):240–242. doi: 10.1038/newbio235240a0. [DOI] [PubMed] [Google Scholar]
  16. Mason S., Warner N. L. The immunoglobulin nature of the antigen recognition site on cells mediating transplantation immunity and delayed hypersentivity. J Immunol. 1970 Mar;104(3):762–765. [PubMed] [Google Scholar]
  17. Nossal G. J., Warner N. L., Lewis H., Sprent J. Quantitative features of a sandwich radioimmunolabeling technique for lymphocyte surface receptors. J Exp Med. 1972 Feb 1;135(2):405–428. doi: 10.1084/jem.135.2.405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Papamichail M., Brown J. C., Holborow E. J. Immunoglobulins on the surface of human lymphocytes. Lancet. 1971 Oct 16;2(7729):850–852. doi: 10.1016/s0140-6736(71)90224-8. [DOI] [PubMed] [Google Scholar]
  19. Parkhouse R. M., Askonas B. A. Immunoglobulin M biosynthesis. Intracellular accumulation of 7S subunits. Biochem J. 1969 Nov;115(2):163–169. doi: 10.1042/bj1150163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Perkins W. D., Karnovsky M. J., Unanue E. R. An ultrastructural study of lymphocytes with surface-bound immunoglobulin. J Exp Med. 1972 Feb 1;135(2):267–276. doi: 10.1084/jem.135.2.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Preud'homme J. L., Klein M., Verroust P., Seligmann M. Immunoglobulines monoclonales de membrane dans les leucémies lymphoïdes chroniques. Rev Eur Etud Clin Biol. 1971 Dec;16(10):1025–1031. [PubMed] [Google Scholar]
  22. Raff M. C. Surface antigenic markers for distinguishing T and B lymphocytes in mice. Transplant Rev. 1971;6:52–80. doi: 10.1111/j.1600-065x.1971.tb00459.x. [DOI] [PubMed] [Google Scholar]
  23. Schrek R. Ultrastructure of blood lymphocytes from chronic lymphocytic and lymphosarcoma cell leukemia. J Natl Cancer Inst. 1972 Jan;48(1):51–64. [PubMed] [Google Scholar]
  24. Sherr C. J., Baur S., Grundke I., Zeligs J., Zeligs B., Uhr J. W. Cell surface immunoglobulin. 3. Isolation and characterization of immunoglobulin from nonsecretory human lymphoid cells. J Exp Med. 1972 Jun 1;135(6):1392–1405. doi: 10.1084/jem.135.6.1392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Smith R. S., Longmire R. L., Reid R. T., Farr R. S. The measurement of immunoglobulin associated with human peripheral lymphocytes. J Immunol. 1970 Feb;104(2):367–376. [PubMed] [Google Scholar]
  26. Vitetta E. S., Baur S., Uhr J. W. Cell surface immunoglobulin. II. Isolation and characterization of immunoglobulin from mouse splenic lymphocytes. J Exp Med. 1971 Jul 1;134(1):242–264. doi: 10.1084/jem.134.1.242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Vitetta E. S., Bianco C., Nussenzweig V., Uhr J. W. Cell surface immunoglobulin. IV. Distribution among thymocytes, bone mrrow cells, and their derived populations. J Exp Med. 1972 Jul 1;136(1):81–93. doi: 10.1084/jem.136.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. WALFORD R. L., GALLAGHER R., TROUP G. M. HUMAN LYMPHOCYTE TYPING WITH ISOLOGOUS ANTISERA; TECHNICAL CONSIDERATIONS AND A PRELIMINARY STUDY OF THE CYTOTOXIC REACTION SYSTEM. Transplantation. 1965 May;3:387–401. doi: 10.1097/00007890-196505000-00009. [DOI] [PubMed] [Google Scholar]
  29. Wilson J. D., Nossal G. J. Identification of human T and B lymphocytes in normal peripheral blood and in chronic lymphocytic leukaemia. Lancet. 1971 Oct 9;2(7728):788–791. doi: 10.1016/s0140-6736(71)92741-3. [DOI] [PubMed] [Google Scholar]
  30. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]
  31. Zucker-Franklin D., Berney S. Electron microscope study of surface immunoglobulin-bearing human tonsil cells. J Exp Med. 1972 Mar 1;135(3):533–548. doi: 10.1084/jem.135.3.533. [DOI] [PMC free article] [PubMed] [Google Scholar]

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