FIG. 1.

The predicted protein product of ORF sso2387 contains sequence features characteristic of eukaryotic protein kinases. Shown is the DNA-derived amino acid sequence of SsoPK2, the predicted protein product of ORF sso2387 from the genome of S. solfataricus (57) (GenBank accession no. AE006641). The positions of plausible candidates for the conserved sequence motifs, called subdomains, characteristic of members of the eukaryotic superfamily of protein kinases are indicated, with key residues highlighted in bold type (see Table 1, footnote a). The first residue of the truncated catalytic domain construct utilized in these studies is indicated by an asterisk underneath. The regions corresponding to the putative phosphopeptides identified by mass spectrometry (see Table 3) are underlined.